As of World 2 and 5, it takes inside mountains (cave levels).

World 1 is a grass plain world, World 3, 6 and 7 are more snowy, while World 4 takes place on lakes and small islands. All of them have 9 normal levels and 1 bonus level. The Dream World consists of over 9 worlds. It also contains an unlockable level as a remake of all of the levels from The Great Giana Sisters. The world map allows the stages to be replayed after being completed. The Soda Bottle and the Bubble Gum are the new objects which are called 'gadgets' that can be collected throught a vending machine instead of Bonus Blocks. The Fire Wheel is the only recurring power-up and also merged to the Lightning (which mean if Giana collect a Fire Wheel, she will be able to breaks blocks and shoots fire balls). Unlike it previous game, the number of power-ups has been reduced. Despite having the same gameplay as The Great Giana Sisters and its similarity of Super Mario Bros., it has a new touch screen and microphone abilities allowing Giana to use various gadgets. Giana Sisters DS contains over 80 levels to be explored for hitting blocks, collecting diamonds, finding secrets and completing levels. You guide Giana through her biggest adventure and help her explore this strange dream. She started to collect her diamonds and then decided to find out more about the secret of her magic treasure chest. Giana's diamonds were scattered all about her. She suddenly found herself in a magical world. Giana, awakened by the light (and the noise), leaped off the bed and followed her prized diamonds into the darkness. Giana's sparkling blue diamonds spilled out of the treasure chest and disappeared into a deep, black hole.

Vibrating with mystical energy, the treasure chest fell off the bed with a crash. As she fell into a deep sleep, magical powers emerged from the treasure chest, bathing Giana's bedroom in a brilliantly bright light. Giana is a young girl who fell asleep one evening while admiring her precious treasure chest. Giana Sisters DS is also Armin Gessert's last game. It is now owned by Black Forest Games following Spellbound's defunct status in 2012 and ownership is shared between the studio and THQ Nordic. There's also a HD port called ' Giana Sisters 2D' for the iPhone, iPad, Android devices, OUYA and Windows systems via Steam. It is a platform game developed by Spellbound Entertainment and available for the Nintendo DS. Giana Sisters DS is the second official sequel in the Giana Sisters series, although it may be also seen as a remake.

The box art of the game (North American version)